Fig. 2

Histopathological findings of specimens in patient A (a1–a4), patient B (b1–b4), patient C (c1–c4), patient D (d1–d4), and patient E (e1–e4). At 10 × magnification, 100 μm, fibrotic tissue (FT), and necrotic bone (NB) can be seen (a3). At magnification 20x, 50 μm, pyknotic osteocytes, and empty lacunae are observed (yellow arrows, a4). At 20 × magnification, 50 μm, osteoblast (black arrow) is detached from the NB (yellow arrow, b3). At 20 × magnification, 50 μm, the fibrous tissue can be seen filled by a plethora inflammatory cells. An oblique view of the venule can also be seen in this section (b4). Fibrotic tissue filled by inflammatory cells can be seen at magnification 2x, 500 μm (c1), and at 5 × magnification, 200 μm (c2). NB with empty lacunae and no sign of osteoblast can be seen at 5 × magnification, 200 μm (c3) and 20 × magnification, 50 μm (c4). Sequestrum/NB, 5 × magnification, 200 μm (d2), fibrotic tissue, 5 × magnification, 200 μm (d3), and inflammatory cells occupy the soft tissue at 20 × magnification, 50 μm (d4). At 20 × magnification, 50 μm, necrotic soft tissue shows the swelling of desmosome in the epithelium (e3) and fibrotic loose connective tissue (e4)