Table 1 Detailed information of primers
Gene name | Sequence | Product size (bp) | Tm (°C) |
|---|---|---|---|
Osteocalcin | AGAGACCCAGGCGCTACCT | 259 | 62.3 |
CTGGGAGGTCAGGGCAAG | 59.33 | ||
Osteopontin | GGTCACTGATTTTCCCACGG | 274 | 58.83 |
GTCCTTCCCACGGCTGTC | 60.05 | ||
Osteonectin | TGGAGGCAGGAGACCACC | 271 | 60.61 |
TCCTTCTGCTTGATGCCG | 57.38 | ||
VEGF | CCACCATGCCAAGTGGTC | 307 | 58.31 |
CATCTCTCCTATGTGCTGGC | 57.83 | ||
Alkaline phosphatase-1 | TTTGGTGGATACACCCCC | 176 | 56.05 |
GCCTGGTAGTTGTTGTGAGC | 59.12 | ||
GAPDH | ACTGGCATGGCCTTCCGT | 289 | 61.65 |
CCACCCTGTTGCTGTAGCC | 60.68 |