Fig. 1
From: Angiogenesis in newly regenerated bone by secretomes of human mesenchymal stem cells
The secretomes of bone marrow-derived mesenchymal stem cells (MSC-CM) promoted tube formation of HUVECs. a Tube formation of HUVECs was compared in AM, and in AM with MSC-CM, VEGF (10 ng/mL), and MSC-CM plus anti-VEGF antibody (10 μg/ml). After 11 days, developing new blood vessels were observed under a microscope and photographed. AM with MSC-CM or EGF stimulated tube formation (scale bar: 500μm). b The total length of blood vessels was analyzed using angiogenesis-measuring software. c The number of capillary connections was also analyzed. There were statistically significant differences between the lengths of blood vessels in the MSC-CM group and those in the other groups although there was no significant difference in the number of joints between MSC-CM and MSC-CM plus anti-VEGF group. (n = 10 for each group). Data are presented as mean ± standard deviation. *P < 0.05, **P < 0.01