The effects of proteins released from silk mat layers on macrophages

Maxillofacial Plastic and Reconstructive Surgery

Table 1 Summary of microarray results

Gene	Observation	Layer 1	Layer 2	Layer 3	Layer 4
Fgf2	2 h	− 0.731	0.450	−1.961*	− 0.247
Fgf2	8 h	− 2.731*	− 3.140*	− 0.591	− 2.441*
Fgf7	2 h	0.483	− 0.720	0.180	0.802
Fgf7	8 h	1.772	− 0.001	0.575	0.640
Igf1r	2 h	− 3.106*	− 3.101*	− 2.943*	− 2.953*
Igf1r	8 h	0.087	0.067	− 0.002	0.246
Col4a1	2 h	− 0.087	− 0.083	0.048	0.066
Col4a1	8 h	0.364	2.300*	2.145*	0.484
Fgfr2	2 h	− 3.857*	− 4.213*	− 3.848*	− 4.119*
Fgfr2	8 h	− 0.126	− 0.067	− 0.157	0.157
Hif1a	2 h	1.435*	− 0.641	0.023	0.419
Hif1a	8 h	− 0.023	− 0.599	− 0.518	− 0.817
Il1b	2 h	3.001*	0.067	0.269	3.102*
Il1b	8 h	3.233*	− 0.308	− 0.107	2.993*
Il6	2 h	5.252*	0.250	0.162	5.356*
Il6	8 h	5.676*	0.058	− 0.080	5.085*
Il19	2 h	1.236*	− 0.134	− 0.168	1.298*
Il19	8 h	3.463*	− 0.054	0.106	3.106*
Mmp3	2 h	− 0.864	− 2.123*	− 2.006*	− 0.511
Mmp3	8 h	2.714*	− 0.113	0.436	1.954*
Mmp9	2 h	1.630*	0.173	− 0.063	1.514*
Mmp9	8 h	1.171*	− 0.195	− 0.162	1.021*
Pdgfb	2 h	2.385*	0.166	0.241	2.155*
Pdgfb	8 h	2.455*	− 0.218	− 0.137	2.021*
Tnfa	2 h	1.462*	0.086	0.026	1.301*
Tnfa	8 h	1.443*	− 0.170	− 0.110	1.182*
Tnfaip3	2 h	2.231*	− 0.084	− 0.254	2.119*
Tnfaip3	8 h	3.420*	0.378	0.446	2.957*
Wnt3a	2 h	4.117*	3.730*	0.666	− 0.270
Wnt3a	8 h	− 3.989*	− 4.493*	− 3.257*	− 3.897*
Wnt7b	2 h	3.949*	2.108*	4.070*	2.147*
Wnt7b	8 h	− 0.630	− 0.570	− 0.704	1.458*

*P < 0.05 when compared to the saline-treated control
Fgf fibroblast growth factor, Col collagen, Igf insulin-like growth factor, Hif hypoxia-inducible factor, Il interleukin, Mmp matrix metalloproteinase, Pdgf platelet-derived growth factor, Tnf tumor necrosis factor