EC is a very rare tumor in the SGs and is present in less than 1.1% of all epithelial SG neoplasia [3,4,5]. Most ECs have been known to be involved in major SGs including the parotid gland and do not often occur in minor SGs such as the lip mucosa. The final diagnosis of EC could be determined under routine microscopic examination where the malignancy is composed of an inner layer of ductal cells surrounded by a layer of clear myoepithelial cells, as seen in this presented case. Cytokeratin positivity in epithelial cells and clear myoepithelial cells can confirm the EC diagnosis [6], and this case also exhibited keratinized tumor epithelium floating in the lymphoid stroma only in the epithelial layer and not into the adjacent fibromuscular adipose tissue (Fig. 3). The differential diagnosis of EC composed of predominantly clear cells includes actinic cell adenocarcinoma, mucoepidermoid carcinoma, oncocytoma, and clear cell adenocarcinoma of MSGs [3,4,5]. There are no definite treatment guidelines for EC in the lips, but surgical excision with a wide resection margin could be recommended in cases involving major SG.
Comedo-type necrosis has been called as comedonecrosis, which is the central luminal inflammation with necrotic cells. This occurred usually in the breast cancer such as intraductal carcinoma or ductal carcinoma in situ; the breast duct is completely plugged by cancer cells. Solid or comedo growth patterns are high-grade ductal carcinoma in situ, if there is a corresponding variation in nuclei or evidence of necrosis. Like those of in our case (Fig. 2), high-grade nuclei of the tumor cells with frequent mitoses, abundant comedo-type necrosis, focal areas of concomitant squamous differentiation, consistent immunoreactivity for cytokeratins [7], and the basaloid tumor cells exhibited relatively undifferentiated cellular characteristics and undeveloped cell organelles in ultrastructural findings. The breast and salivary glands are both exocrine glands sharing similar morphologic features; thus, the salivary gland metaplasia could appear as a diffuse adenosis-like lesion in the breast, and the presence of benign salivary-type acini and ducts in the breast without accompanying salivary gland-type tumors had been reported [8].
AC occurs in patients over the age of 45, with a male predominance in a 10:1 ratio [9], and AC more often affects the lower lip versus the upper lip. AC is known to be caused by chronic sunlight exposure and ultraviolet radiation from outdoor lifestyles such as farming, sailing, fishing, windsurfing, mountaineering, and golfing, giving AC the name of sailor’s lip or farmer’s lip [1, 9]. Most AC patients complain of painless recurring symptoms including hard and dry sensations or cracking lip mucosal sensations. Diverse appearances of AC include edematous reddish in the acute stage and grey-whitish or dried hyperkeratotic wrinkled lesions in the chronic stage for several months or even years (Fig. 4) [10].
The differential diagnosis of AC includes actinic lichen planus (ALP), herpes simplex lesions, exfoliative cheilitis, contact cheilitis (CC), autoimmune blistering disease including pemphigus vulgaris (PV), lichenoid drug eruptions, cheilitis granulomatosa (CGM), cheilitis grandularis (CGL), and early carcinoma in situ (CIS) [11, 12]. ALP is a variant of OLF occurring in sunlight-exposed areas in patients with dark skin, and sunlight in lip LP has been suggested in lower lip involvement with male predominance. Typical histologic findings include lichenoid lymphocytic infiltrates with interface vacuolar changes and focal subepithelial blistering [12]. Exfoliative cheilitis exhibits hyperkeratosis and erythema in the histologic findings, which is associated with a history of factitial injury [9, 13]. CC is triggered by an allergy to toothpaste or beauty care products or an irritation to extremely hot, cold, and dry weather and characterized by scaling and erythema along the vermillion border of the lips with sloughing of the surface epithelium [11, 13]. PV is characterized by blisters, erosion, and an ulcer involving the lips and buccal mucosa. More than 85% of cases of oral PV are preceded by a cutaneous lesion [11, 14]. CGM is a rare condition manifesting as an episodic, non-tender enlargement of one or both lips and feels firm and nodular upon palpation [11, 15]. CGM could be considered with CGL as a potential predisposing factor for development of AC and SCC. CGL is a chronic inflammatory condition manifesting as MSG hypersecretion with ductal ectasia with swollen lips, nodular growth with everted margins, and ulceration [10, 11]. CGL is characterized by progressive enlargement and eversion of the lower labial mucosa that results in obliteration of the mucosal-vermilion interface [10, 11].
In this case, if the lower lip was diagnosed with premalignant AC or CGL, malignant transformation to EC could have been prevented with early management. If we know the clinical diagnosis entity as AC, wide V-shaped resection could be avoided. To prevent AC, chronic habits and irritation and sunlight exposure must be controlled and avoided. More active treatment options for AC include cryosurgery, electrosurgery, carbon dioxide laser vaporization, scalpel vermilionectomy, and chemical peel with 5-fluorouracil (5-FU), trichloroacetic acid (TCA), or imiquimod (INN) (Fig. 4).
Cryosurgery is the first treatment of choice for AC through application of liquid nitrogen to the lesion, with a cure rate greater than 96%. Electrosurgery is also a useful treatment method under local anesthesia, but could delay healing with scar formation of the adjacent tissue [1, 16]. Carbon dioxide laser vaporization with or without scalpel vermilionectomy could be used in broad or recurring AC and removes the entire vermillion border, leaving the underlying muscle intact. Although a linear scar may form, these techniques have been found to be effective and useful for surgical management of AC. Topical 5-FU is known to be effective against the minor form of AC by blocking DNA synthesis and having less of a negative effect on normal skin or lip mucosa. Complete remission has been reported in 50% of cases of AC after 2 to 4 weeks of application, with minimal scarring [1]. Topical TCA has been also used in 50% concentration form, as in the case of topical chemoablative application for genital warts or tattoo removal, but its limited reports have shown a low complete remission rate less than 30% [1, 17]. INN is one of the representative chemical peel agents for AC and promotes immune response induction of apoptosis of tumor cells. Complete remission of actinic keratosis has been seen in more than 45% of patients, but the long-term effects with the effective dose or duration has not been clearly identified in AC management [1].